agarosegelelektrofores
Agarosegel electrophoresis is a laboratory technique used to separate nucleic acid molecules, primarily DNA, by size. A thin slab of agarose, a polysaccharide derived from seaweed, is melted in a buffer solution and poured into a casting tray to form a uniform gel. Once solidified, wells are cut into the gel where small volumes of DNA sample are loaded.
The gel is placed in an electrophoresis chamber filled with running buffer. An electric field is applied
Typical agarose concentrations range from 0.5 % to 2 % for DNA fragments between a few hundred base pairs
Key advantages include gentle handling of DNA because the gel matrix prevents shear forces, and the ability