WST1assays
WST1 assays are a type of colorimetric assay used to measure cell viability, proliferation, and cytotoxicity. The assay relies on the cleavage of the tetrazolium salt WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate) by mitochondrial dehydrogenases in viable cells. This cleavage results in the formation of a yellow-colored formazan dye, which is soluble in cell culture medium and can be quantified spectrophotometrically at a wavelength of approximately 450 nm.
WST1 assays are widely used in biomedical research due to their simplicity, sensitivity, and compatibility with
The procedure typically involves adding WST1 reagent to cell cultures, incubating for a defined period, and
One advantage of WST1 assays is their non-destructive nature when used with short incubation times, enabling
WST1 assays are commercially available as kits, often including optimized reagents and protocols, and are compatible