Täppsekveneerimine

Täppsekveneerimine, also known as targeted sequencing, is a molecular biology technique used to sequence specific regions of DNA or RNA within a larger genome. Instead of sequencing the entire genome, which can be time-consuming and expensive, täppsekveneerimine focuses on predefined areas of interest. This approach is particularly useful when researchers are looking for mutations in specific genes, variations in a particular pathway, or the presence of known genetic markers.

The process typically involves identifying the target regions, designing probes or primers that bind specifically to these regions, and then enriching these targets from a DNA or RNA sample. This enrichment step ensures that the subsequent sequencing reaction primarily analyzes the desired sequences. Once enriched, the samples are then subjected to standard DNA sequencing technologies, such as next-generation sequencing (NGS).

The advantages of täppsekveneerimine include its cost-effectiveness and speed compared to whole-genome sequencing. It allows for higher depth of coverage in the targeted regions, which can improve the accuracy of variant detection. This makes it a valuable tool in various applications, including clinical diagnostics for genetic diseases, cancer research for identifying driver mutations, and agricultural applications for trait selection. However, it is important to note that täppsekveneerimine will not detect variants outside of the predefined target regions.