Transcriptasa
Transcriptasa is a term used in molecular biology to describe enzymes that catalyze nucleic acid synthesis using a template. In practice, it is most commonly encountered as reverse transcriptase (RT), an enzyme that synthesizes DNA from an RNA template. The DNA product (cDNA) can then be amplified and analyzed by PCR. In a broader sense, transcriptasa can also refer to DNA-dependent RNA polymerases that drive cellular transcription, synthesizing RNA from a DNA template.
Biology and mechanism: Reverse transcriptases typically require divalent metal ions, catalyze polymerization in the 5' to
Applications and significance: Reverse transcriptases are essential for converting RNA into DNA for gene expression studies,