RNaseOUT

RNaseOUT is a protein that acts as a ribonuclease inhibitor. Ribonucleases, or RNases, are enzymes that degrade RNA molecules. RNaseOUT is specifically known to inhibit certain RNases, such as RNase A, by binding to their active sites. This inhibition prevents the RNases from cleaving RNA.

The function of RNaseOUT is crucial in molecular biology techniques where maintaining the integrity of RNA is essential. For example, in experiments involving RNA isolation, reverse transcription, or RNA sequencing, the presence of RNases can lead to the degradation of valuable RNA samples. RNaseOUT, or similar inhibitors, are often added to reaction buffers to protect RNA from enzymatic degradation.

RNaseOUT is a naturally occurring protein found in various organisms. Its mechanism of action involves forming a stable complex with target RNases, rendering them inactive. This binding is typically non-covalent and reversible, although the stability of the complex can vary depending on the specific RNase and the conditions. The protein's discovery and characterization have contributed to a better understanding of RNA stability and the development of more effective RNA handling protocols. Researchers utilize RNaseOUT as a tool to ensure the success of RNA-based experiments, safeguarding against the loss of genetic information.