RNAintegriteit
RNA integrity refers to the state of intactness of RNA molecules within a sample. It is a crucial factor in the success of many molecular biology applications, particularly those involving RNA analysis, such as gene expression profiling, RNA sequencing, and quantitative reverse transcription polymerase chain reaction (RT-qPCR). Degraded RNA, where the phosphodiester bonds have been broken, can lead to inaccurate and unreliable experimental results.
The integrity of RNA is commonly assessed using techniques like gel electrophoresis or automated capillary electrophoresis.
Factors that can compromise RNA integrity include the presence of RNases (ribonucleases), which are enzymes that