RNAaliquotit

RNAaliquotit are small, measured portions of RNA extracted from biological samples. These aliquots are used to preserve the integrity of the nucleic acid and to enable reproducible downstream analyses such as quantitative polymerase chain reaction (qPCR), next‑generation sequencing, microarray hybridization, and enzyme‑linked assays. Because RNA is intrinsically unstable and highly susceptible to enzymatic degradation, aliquoting minimizes freeze‑thaw cycles and the associated loss of quality. By dividing a single extraction into multiple aliquots, researchers can perform replicate experiments, run controls, and preserve material for future studies without contaminating the primary sample.

Standard practice for preparing RNAaliquotit involves first assessing RNA reliability through spectrophotometric ratios (A260/280 and A260/230) and using electrophoresis or capillary systems to gauge RNA integrity by calculating an RNA integrity number (RIN). Once deemed acceptable, the total RNA is distributed into tubes of predetermined volumes—commonly 10 µL, 50 µL, or 100 µL—depending on the anticipated application. The aliquots are immediately frozen at –80 °C or stored in liquid nitrogen for long‑term preservation. To further protect against degradation, cryoprotectants such as guanidinium thiocyanate can be added, and care is taken to avoid repeated exposure to RNases.

RNAaliquotit also facilitate compliance with best laboratory practices, including traceability, batch tracking, and documentation of handling. In multi‑center collaborative projects, sharing aliquots allows each participant to conduct independent assays while maintaining consistency across datasets. Additionally, by creating a master stock and sub‑aliquots, researchers can rescue experiments that would otherwise fail due to sample exhaustion. Overall, RNAaliquotit represent a critical step in RNA‑based research, optimizing both sample integrity and experimental reproducibility.