RIPCLIP

RIPCLIP, or RNA Interference by Programmable CRISPR-Cas9, is a molecular biology technique that combines the CRISPR-Cas9 system with RNA interference (RNAi) to target and degrade specific RNA sequences. This method leverages the Cas9 protein, which is guided by a synthetic guide RNA (gRNA) to bind to a target RNA sequence. Once bound, the Cas9 protein creates a double-stranded break in the RNA, which is then recognized and degraded by the cellular machinery. RIPCLIP is particularly useful for studying gene expression and function, as it allows for the specific degradation of target RNAs, thereby preventing their translation into proteins. This technique has been employed in various biological systems, including yeast, plants, and mammals, to investigate the roles of specific RNAs in cellular processes. RIPCLIP has also been adapted for use in vivo, enabling researchers to study RNA function in living organisms. The method's precision and versatility make it a valuable tool in the field of molecular biology and genetics.