Pöördfaasvedel

Pöördfaasvedel refers to a type of stationary phase used in high-performance liquid chromatography (HPLC). In HPLC, the stationary phase is a solid material packed into a column, and the mobile phase is a liquid that flows through the column. The separation of analytes is based on their differential interactions with the stationary and mobile phases.

In normal-phase chromatography, the stationary phase is polar and the mobile phase is nonpolar. In reversed-phase chromatography, the stationary phase is nonpolar and the mobile phase is polar. Pöördfaasvedel specifically denotes the mobile phase used in reversed-phase HPLC.

Commonly, the mobile phase in reversed-phase HPLC consists of a mixture of water or an aqueous buffer and a water-miscible organic solvent. Typical organic modifiers include acetonitrile, methanol, and isopropanol. The ratio of water to organic solvent is a crucial parameter that influences the separation. Increasing the organic modifier content generally leads to faster elution of analytes because it reduces their interaction with the nonpolar stationary phase.

The choice of buffer and pH in the aqueous component is also important, especially for ionizable compounds, as it affects their charge state and, consequently, their retention. Pöördfaasvedel plays a critical role in achieving effective separation and resolution of compounds based on their hydrophobicity.