NADNADHtiloilla

NADNADHtiloilla is a provisional genus of microorganisms identified from anaerobic environments and represented primarily by genomic data rather than a cultivated strain. The name reflects the central role of the NAD+/NADH redox couple in the lineage’s proposed metabolism and serves as a placeholder until formal description and isolation are published. Current taxonomic placement is provisional, with analyses based on metagenomic data suggesting affiliation with Bacteria, but lacking a cultured representative to confirm higher-level classification.

Sequences associated with NADNADHtiloilla have been recovered from anoxic sediment in freshwater wetlands and from enrichment cultures designed to select for NADH-dependent respiration. The lineage appears to be unicellular and is represented by draft genomes that indicate a relatively compact genetic content, including clusters of genes involved in redox balance and NADH metabolism.

Metabolism and genomics: Genomes attributed to NADNADHtiloilla encode enzymes characteristic of NADH dehydrogenases and NAD+-dependent dehydrogenases, consistent with a metabolism centered on NADH generation and consumption. The organism is hypothesized to be fermentative or anaerobically respiring, using NADH as an electron donor in the reduction of alternative electron acceptors while regenerating NAD+ for continued carbon flux. The exact substrates, electron acceptors, and energy-yielding pathways remain to be validated in culture.

Etymology and significance: The name derives from the NAD/NADH coenzymes and from a genus-like suffix chosen to reflect its taxonomic placeholder status. If isolated and described, NADNADHtiloilla could illuminate NADH metabolism in anaerobes and inform biotechnological approaches to redox balancing and NADH recycling.