KinaseGlo

KinaseGlo is a luminescent assay technology developed by Promega Corporation for detecting and quantifying kinase activity. It is based on the principle of bioluminescence resonance energy transfer (BRET) and a specialized luciferase enzyme. The assay typically involves a substrate that, when phosphorylated by a kinase, undergoes a conformational change. This change allows for energy transfer from a donor luciferase to an acceptor fluorescent protein, resulting in light emission. The intensity of this emitted light is directly proportional to the level of kinase activity in a sample.

The KinaseGlo assay offers several advantages, including high sensitivity, a broad dynamic range, and compatibility with both purified kinases and cell-based assays. It can be used in various applications such as drug discovery, kinase profiling, and screening for kinase inhibitors. The technology allows for the assessment of both endogenous and overexpressed kinases in a homogeneous format, meaning no washing steps are typically required, which streamlines experimental workflows. Promega provides a range of KinaseGlo kits tailored for different kinase families and applications, simplifying assay setup and optimization for researchers. The assay is also amenable to high-throughput screening due to its speed and ease of use.