Gelpermetiechromatografie

Gelpermetiechromatografie, also known as gel permeation chromatography (GPC) or size-exclusion chromatography (SEC), is a technique used to separate molecules based on their size. It is commonly employed in the fields of biochemistry, polymer science, and materials science. The method involves passing a mixture of molecules through a column filled with a porous gel matrix. The gel matrix allows smaller molecules to pass through its pores, while larger molecules are excluded and elute earlier. The separation is based on the hydrodynamic volume of the molecules, which is related to their size and shape. GPC is particularly useful for determining the molecular weight distribution of polymers and for purifying biomolecules. The technique can be performed using various solvents and is compatible with a wide range of sample types. GPC is a versatile and widely used analytical tool in the characterization and purification of complex mixtures.