Fosforotioaattilinkkejä
Fosforotioaattilinkkejä, also known as phosphorothioate linkages, are a modified internucleotide bond in nucleic acids. In a natural phosphodiester bond, a phosphate group links two sugar molecules. In a phosphorothioate linkage, one of the non-bridging oxygen atoms in the phosphate group is replaced by a sulfur atom. This substitution significantly alters the chemical properties of the linkage.
The introduction of sulfur into the phosphate backbone makes the linkage more resistant to nuclease degradation.
Oligonucleotides containing phosphorothioate linkages are commonly employed as antisense agents and small interfering RNAs (siRNAs). These
The synthesis of phosphorothioate linkages can be achieved through various chemical methods, typically involving the oxidation