Elektroporációval

Elektroporációval, sometimes referred to as electropermeabilization, is a laboratory technique used to increase the permeability of cell membranes by applying an electric field. This method creates temporary pores in the lipid bilayer of the cell, allowing molecules that would not normally pass through, such as DNA, RNA, proteins, or drugs, to enter the cell.

The process involves placing cells in a solution containing the substance to be delivered. This suspension is then subjected to brief, high-voltage electric pulses. These pulses cause a rapid change in the electrical potential across the cell membrane, leading to the formation of transient pores. Once the electric pulse ceases, the cell membrane reseals, and the introduced molecules are now inside the cell.

Elektroporációval has found widespread applications in various fields. In molecular biology and genetic engineering, it is a crucial tool for gene transfection, enabling the introduction of foreign genes into cells for research purposes or for the development of gene therapies. In drug delivery, it is being explored as a method to enhance the uptake of therapeutic agents into target cells, potentially improving treatment efficacy and reducing side effects. Furthermore, it is utilized in diagnostics and in the production of certain biologics. The effectiveness of elektroporációval can be influenced by factors such as pulse duration, intensity, waveform, and the composition of the buffer solution.