DenovoSequenzierung

DenovoSequenzierung, also known as de novo sequencing, is a method used in molecular biology to determine the exact nucleotide sequence of a DNA or RNA molecule without relying on a pre-existing reference sequence. This process is essential when studying organisms that have not been previously sequenced or when analyzing novel genetic material. Unlike resequencing, which aligns newly obtained sequences to a known genome, denovoSequenzierung builds the sequence from scratch by assembling short, overlapping reads generated by sequencing technologies.

The process typically involves fragmenting the DNA or RNA into smaller pieces, sequencing each fragment to obtain short reads, and then using computational algorithms to piece these reads back together. These algorithms identify overlapping regions between the reads and order them to reconstruct the original, longer sequence. The accuracy of denovoSequenzierung depends heavily on the length and quality of the reads, as well as the complexity of the genome or transcriptome being analyzed.

DenovoSequenzierung is critical for a variety of applications, including the identification of new genes, the study of microbial communities, the characterization of viral genomes, and the investigation of genetic variations in newly discovered species. It plays a fundamental role in genomics research, enabling scientists to explore the genetic makeup of organisms and gain insights into their biological functions and evolutionary history.