DARPin
DARPin stands for designed ankyrin repeat protein, a class of engineered binding proteins derived from ankyrin repeat motifs. DARPins are designed to recognize target proteins with high affinity and specificity, offering an antibody-like binding surface without using immunoglobulin frameworks. The technology was developed in the late 1990s and early 2000s, with key contributions from Albert Plückthun and colleagues, and has since been pursued by research groups and biotechnology companies.
Structure and properties: A DARPin consists of a series of ankyrin repeat units arranged in a modular,
Generation and selection: Diversity is introduced in the binding loops of the ankyrin repeats, and target-binding
Applications and formats: DARPins are explored for therapeutics, diagnostics, and research tools. They can be used