ChainTerminationMethode
ChainTerminationMethode is a DNA sequencing method used to determine the order of nucleotides in a DNA molecule. It relies on controlled DNA synthesis in which chain termination events mark the end of growing strands, allowing the construction of a set of fragments that encode the sequence information.
The principle centers on incorporating chain-terminating nucleotides, known as dideoxynucleotides (ddNTPs), which lack the 3' hydroxyl
Historically, four reactions were run in parallel and the terminated fragments were separated by polyacrylamide or
ChainTerminationMethode played a pivotal role in early DNA sequencing and remains a foundational reference in molecular