Bioanalysen

Bioanalysen, also known as bioanalysis, is the quantitative determination of xenobiotics (foreign chemical substances) and biogenic substances (natural chemical substances) in biological matrices. This field is crucial for understanding drug metabolism, pharmacokinetics, and the effects of various substances within living organisms. It encompasses a wide range of analytical techniques applied to biological samples such as blood, urine, tissue, and saliva.

The primary goal of bioanalysis is to accurately measure the concentration of a substance of interest. This is essential for drug development, where bioanalytical studies help determine the absorption, distribution, metabolism, and excretion (ADME) profile of potential drug candidates. This information guides dosage regimens and assesses the safety and efficacy of new medications. Beyond pharmaceuticals, bioanalysis plays a vital role in clinical diagnostics, toxicology, forensic science, and environmental monitoring.

Various analytical techniques are employed in bioanalysis, with chromatography and mass spectrometry being the most prominent. Liquid chromatography (LC) coupled with mass spectrometry (MS), often referred to as LC-MS, is a gold standard for its sensitivity, selectivity, and ability to identify and quantify analytes in complex biological matrices. Other methods include gas chromatography (GC), immunoassays, and capillary electrophoresis.

Method validation is a critical aspect of bioanalysis. It ensures that the analytical method used is reliable, reproducible, and fit for its intended purpose. This involves assessing parameters such as accuracy, precision, selectivity, sensitivity, and stability of the analyte in the biological matrix. Robust bioanalytical data is paramount for regulatory submissions and scientific publications.