Bindingstests
Binding tests, or binding assays, are laboratory procedures that quantify the interaction between two or more molecules, most commonly a ligand and a molecular target such as a protein, receptor, nucleic acid, or cell surface molecule. They are used to measure affinity, specificity, kinetics, and occupancy, and to characterize molecular interactions for research, diagnostics, and drug development.
Common formats include direct binding assays, where a labeled ligand binds to the target; competitive binding
Key parameters derived from binding data include the dissociation constant (Kd), maximum binding (Bmax), the inhibition
Typical workflow begins with preparation and characterization of the target, incubation with a detectable ligand under
Applications span drug discovery and pharmacology, where binding affinity informs lead optimization; diagnostics, where binding assays