Home

AOX1

AOX1 is a gene name used for different enzymes in different organisms. In the methylotrophic yeast Pichia pastoris (Komagataella phaffii), AOX1 encodes alcohol oxidase 1, a peroxisomal flavoprotein that initiates methanol utilization. In humans, AOX1 refers to aldehyde oxidase 1, a cytosolic molybdoenzyme that oxidizes aldehydes and heterocycles. These proteins are unrelated and share the AOX1 designation in their respective contexts.

In P. pastoris, the alcohol oxidase 1 enzyme is a peroxisomal, FAD-dependent oxidase that catalyzes the oxidation

Regulation and biotechnology use: The AOX1 promoter is strongly methanol-inducible and is repressed by common carbon

of
methanol
to
formaldehyde,
with
concomitant
production
of
hydrogen
peroxide.
The
enzyme
exists
as
a
homodimer
and
is
targeted
to
peroxisomes,
where
it
participates
in
the
methanol
metabolism
pathway
alongside
other
enzymes
such
as
formaldehyde
dehydrogenase.
The
genome
contains
two
AOX
genes,
AOX1
and
AOX2,
with
AOX1
typically
acting
as
the
major,
methanol-inducible
gene,
and
AOX2
providing
a
smaller
contribution
to
overall
activity.
sources
like
glucose
or
glycerol.
This
promoter
is
widely
used
to
drive
high-level
expression
of
recombinant
proteins
in
P.
pastoris,
making
the
yeast
a
popular
system
for
protein
production.
Expression
strains
are
often
annotated
as
Mut+
when
AOX1
is
functional,
or
engineered
to
alter
AOX1
activity
to
achieve
controlled
induction.
Handling
and
optimization
of
methanol
feeding
are
important
considerations
in
industrial
applications.