virtauscytometria

Virtauscytometria, also known as flow cytometry, is a powerful laboratory technique used to detect and measure physical and chemical characteristics of a fluid sample as it flows through a laser beam. It is a method for counting and sorting cells and other particles based on their light scattering and fluorescence properties. The core principle involves suspending cells in a stream of liquid and passing them one by one through a focused laser. As each cell intersects the laser, it scatters light. Some light scatters forward, providing information about cell size, while other light scatters to the side, offering insights into internal complexity or granularity.

Additionally, cells can be labeled with fluorescent dyes or antibodies conjugated to fluorochromes. When these fluorescent molecules are excited by the laser, they emit light at specific wavelengths. Detectors then capture this emitted fluorescence, allowing for the identification and quantification of specific cell populations based on the expression of particular markers. This enables researchers to analyze multiple parameters simultaneously for thousands of cells per second.

The applications of virtauscytometria are extensive, spanning fields such as immunology, hematology, cell biology, microbiology, and cancer research. It is routinely used for cell counting, cell sorting, immunophenotyping, cell cycle analysis, apoptosis detection, and monitoring of disease progression. Its ability to provide rapid, quantitative, and multiparametric data makes it an indispensable tool in both research and clinical diagnostics.