størrelseeksklusjonskromatografi

størrelseekskl is a Danish term that translates to "size exclusion" in English. It refers to a separation technique used in various scientific disciplines, most commonly in chromatography. This method separates molecules based on their hydrodynamic volume, or effectively their size in solution, rather than their chemical properties.

In size exclusion chromatography (SEC), the stationary phase consists of porous beads. Larger molecules that are too big to enter the pores of the beads will pass through the column more quickly, as they are excluded from a larger portion of the stationary phase. Conversely, smaller molecules will enter the pores and thus spend more time within the stationary phase, leading to a longer retention time. This differential movement allows for the separation of molecules within a sample.

The application of størrelseekskl is widespread. It is frequently employed in the analysis of polymers to determine their molecular weight distribution. In biochemistry, it is used to separate proteins from each other or to determine their molecular mass. It can also be utilized in determining the aggregation state of biomolecules. The effectiveness of the separation depends on the choice of column and the mobile phase, which must be compatible with the molecules being analyzed. This technique is valuable for both analytical and preparative purposes.