spectrophotometrische

Spectrophotometrische refers to methods or techniques that utilize spectrophotometry. Spectrophotometry is a quantitative analytical method that measures the intensity of light as a function of wavelength. It works by passing a beam of light through a sample and measuring the amount of light that is absorbed or transmitted by the sample. This absorption or transmission of light is related to the concentration of the substance in the sample.

The fundamental principle behind spectrophotometry is the Beer-Lambert Law, which states that the absorbance of a solution is directly proportional to the concentration of the absorbing species and the path length the light travels through the solution. Different substances absorb light at different wavelengths, and this characteristic absorption spectrum can be used to identify and quantify substances.

Spectrophotometrische techniques are widely used in various scientific disciplines, including chemistry, biology, environmental science, and materials science. In chemistry, they are employed for determining the concentration of solutions, identifying unknown compounds, and studying reaction kinetics. Biologists use spectrophotometry to measure the concentration of biomolecules like proteins and nucleic acids, and to monitor cellular growth. Environmental scientists utilize it for analyzing water and air quality, detecting pollutants, and assessing the effectiveness of treatment processes. In materials science, spectrophotometrische methods can be used to characterize the optical properties of materials.