sisestandardite

Sisestandardite, often referred to as internal standards in English, are substances added to a sample in a known and fixed amount during analytical chemistry. Their primary purpose is to serve as a reference point for quantifying other components within the same sample. By comparing the signal generated by the analyte of interest to the signal generated by the sisestandard, researchers can determine the concentration of the analyte more accurately.

The effectiveness of a sisestandard relies on several key characteristics. Ideally, it should have chemical and physical properties that are very similar to the analyte being measured, such as similar volatility, solubility, and detector response. However, it must be chemically distinct enough that it does not naturally occur in the sample matrix or interfere with the detection of the analyte. The sisestandard is added to both calibration standards and unknown samples at the same concentration, ensuring that any variations in sample preparation, injection volume, or instrument performance affect both the analyte and the sisestandard equally.

Commonly used sisestandardites include isotopically labeled versions of the analyte, such as deuterium-labeled compounds for mass spectrometry. These labeled compounds behave almost identically to their non-labeled counterparts during analysis but can be distinguished by their mass. Other examples include structurally similar compounds or compounds with known and stable signal intensities. The use of sisestandardites is crucial for improving the accuracy and precision of quantitative analytical methods, particularly when dealing with complex matrices or when high sensitivity is required.