resazurinAlamar

Resazurin is a water-soluble, orange-red, crystalline compound with the chemical formula C20H14N4O5. It is a tetrazolium salt that is commonly used as a redox indicator in biological and chemical assays. Resazurin is reduced to resorufin in the presence of NADH or other reducing agents, resulting in a color change from orange-red to pink. This property makes it useful for monitoring metabolic activity, cell viability, and redox potential in various biological systems.

Resazurin is also known as 3,3',5,5'-tetramethylbenzidine (TMB) and is often used in conjunction with the enzyme horseradish peroxidase (HRP) in ELISA (Enzyme-Linked Immunosorbent Assay) and other colorimetric assays. In these assays, the substrate TMB is oxidized by HRP to produce resazurin, which can then be reduced to resorufin in the presence of reducing agents, resulting in a color change that can be measured spectrophotometrically.

Resazurin has been used in a variety of applications, including the detection of bacterial growth, the measurement of oxygen consumption, and the assessment of cell viability and cytotoxicity. It is also used in the development of biosensors and bioassays for the detection of various analytes, such as glucose, lactate, and hydrogen peroxide.

In summary, resazurin is a versatile redox indicator that is widely used in biological and chemical assays for the monitoring of metabolic activity, cell viability, and redox potential. Its ability to undergo a color change in response to reducing agents makes it a valuable tool in a variety of analytical and diagnostic applications.