referentieoplossing

Referentieoplossing, often translated as reference solution or standard solution, is a precisely prepared chemical solution of known concentration. It is used as a benchmark for quantitative analysis. The preparation of a referentieoplossing involves dissolving a highly pure substance, known as a primary standard, in a solvent. The mass of the primary standard must be accurately weighed, and the final volume of the solution must be precisely measured. Primary standards possess specific characteristics, such as high purity, stability, and a known molecular weight, to ensure the accuracy of the referentieoplossing.

Referentieoplossingen are indispensable in various analytical techniques, including titration, spectrophotometry, and chromatography. In titrations, a referentieoplossing of a known concentration is used to determine the unknown concentration of another substance. By reacting the referentieoplossing with the analyte and observing the equivalence point, the concentration of the analyte can be calculated. Similarly, in spectrophotometry, a referentieoplossing is used to create a calibration curve, which relates absorbance to concentration. This curve then allows for the determination of the concentration of unknown samples. The reliability of analytical results hinges on the accuracy and purity of the referentieoplossing used.