primerisuunnittelun
Primerisuunnittelun, often translated as primer design, is a critical step in polymerase chain reaction (PCR) and related nucleic acid amplification techniques. The goal of primer design is to create short, synthetic DNA sequences, known as primers, that specifically bind to the target DNA sequence. These primers serve as starting points for DNA polymerase to synthesize new DNA strands, effectively amplifying the desired region of interest.
Effective primer design considers several factors. Primers should be of appropriate length, typically between 18 and