microcarrierbased

Microcarrierbased refers to a method of cell culture that utilizes microcarriers as a substrate for cell attachment and growth. Microcarriers are small, inert particles, typically ranging from 50 to 300 micrometers in diameter, that are suspended in a liquid culture medium. These particles provide a larger surface area for cells to adhere to and proliferate, allowing for higher cell densities to be achieved compared to traditional static flask cultures.

The principle behind microcarrierbased cell culture is to mimic the in vivo environment where cells often grow on extracellular matrices or other supporting structures. The microcarriers can be made from various materials, including polystyrene, collagen-coated polymers, or dextran. The choice of material often depends on the specific cell type being cultured and its attachment requirements.

This technique is widely employed in the biopharmaceutical industry for the production of therapeutic proteins, vaccines, and other biological products that require large-scale cell cultivation. It is particularly advantageous for anchorage-dependent cells, which need a surface to grow. Advantages include increased volumetric productivity, easier scale-up, and suitability for automated systems. However, challenges can include potential shear stress on cells due to agitation required for suspension, and the need for specialized equipment for harvesting and downstream processing. The recovery of cells and products from microcarriers can also require specific protocols.