mEGFPs

mEGFP refers to a monomeric enhanced green fluorescent protein. It is a genetically engineered variant of the naturally occurring green fluorescent protein (GFP) found in the jellyfish Aequorea victoria. The "m" designation signifies its monomeric nature, meaning it exists as a single polypeptide chain, which is important for avoiding potential issues associated with multimeric proteins in certain biological applications. The "enhanced" aspect indicates that mEGFP has undergone modifications to improve its properties, such as brighter fluorescence, faster maturation, and increased photostability compared to earlier versions of GFP.

mEGFP is widely used as a reporter protein in molecular biology and cell biology. Researchers fuse mEGFP to other proteins of interest to visualize their localization, expression levels, and dynamics within living cells or organisms. Its strong green emission makes it easily detectable using fluorescence microscopy. The protein itself consists of approximately 238 amino acids and exhibits a characteristic excitation maximum around 488 nm and an emission maximum around 507 nm. Its stability and brightness make it a valuable tool for a variety of research endeavors, including gene expression studies, protein-protein interaction analysis, and tracking cellular processes.