kalibraatioviiva

Kalibraatioviiva, also known as a calibration curve or standard curve, is a fundamental tool in analytical chemistry and other scientific disciplines. It is a graphical representation that relates the measured signal from an analytical instrument to the concentration of a known substance. The process of creating a kalibraatioviiva involves preparing a series of solutions with precisely known concentrations of the analyte of interest. These solutions, called standards, are then analyzed using the instrument, and the corresponding signal (e.g., absorbance, fluorescence, peak area) is recorded for each concentration.

Once the data points are collected, they are plotted on a graph with concentration on the x-axis and the instrument signal on the y-axis. A mathematical function, often a linear regression, is then fitted to these data points to establish the relationship between signal and concentration. This fitted line or curve is the kalibraatioviiva. The equation derived from this fit can then be used to determine the concentration of an unknown sample by measuring its signal and interpolating this value onto the kalibraatioviiva. The accuracy and reliability of analytical results heavily depend on the quality and proper construction of the kalibraatioviiva. It is crucial that the standards used are accurately prepared and that the instrument is functioning correctly during the calibration process.