hematoksiliinieosin

Hematoxylin and eosin, often abbreviated as H&E, is the most widely used histological stain in medical diagnosis and scientific research. It is a double stain that utilizes two dyes, hematoxylin and eosin, to differentiate cellular components. Hematoxylin is a basic dye that stains acidic structures, such as the nucleus, blue to purple. Eosin is an acidic dye that stains basic structures, such as the cytoplasm and extracellular matrix, pink to red. The differential staining allows for clear visualization of tissue morphology, making it an indispensable tool for pathologists to identify abnormalities in cells and tissues, aiding in the diagnosis of diseases like cancer. The preparation of H&E stained slides typically involves fixing tissue samples, embedding them in paraffin, sectioning them thinly, and then immersing them in solutions of hematoxylin and eosin, followed by dehydration and mounting. The precise colors can vary depending on the specific formulation of the dyes and the tissue type, but the general principle of nuclear blue/purple and cytoplasmic pink/red staining remains consistent.