dyeuptake
Dye uptake, occasionally written as dyeuptake, refers to the absorption or binding of a colored dye by a substrate from a solution. It is used to visualize, quantify, or probe properties of biological tissues, cells, microorganisms, and materials. The extent and pattern of uptake depend on the chemical nature of the dye (charge, molecular size, hydrophobicity), the characteristics of the substrate (cell wall composition, membrane integrity, porosity), and the conditions of exposure (pH, temperature, duration).
Mechanisms vary: dyes can diffuse into cells or tissues, bind electrostatically to charged sites, or react covalently
Applications include routine tissue staining and microscopy, identification and differentiation of microorganisms, viability and cytotoxicity testing,
Considerations include dye stability, background staining, washing efficiency, and the need for appropriate controls to distinguish