dCas9based
dCas9-based refers to systems built on catalytically dead Cas9, a variant of the CRISPR-Cas9 nuclease engineered to bind DNA at a sequence specified by a guide RNA without cutting. By disabling the nuclease activity, dCas9 serves as a programmable DNA-binding platform that can recruit effector proteins to targeted genomic loci.
When fused to transcriptional repressors or activators, dCas9 can modulate gene expression in a programmable way,
Applications of dCas9-based systems span functional genomics, gene regulation studies, and high-throughput screens. They enable loss-
Design considerations and limitations include the requirement for a suitable PAM sequence and potential off-target binding,
History and status: dCas9-based tools emerged in the early to mid-2010s and have become widespread in molecular