biotinavidin
Biotin and avidin are proteins with a remarkable affinity for each other. Biotin, also known as vitamin B7, is a water-soluble vitamin crucial for various metabolic processes in living organisms. Avidin is a protein found in egg whites and other sources. The binding interaction between biotin and avidin is one of the strongest non-covalent interactions known in nature, with an association constant in the femtomolar range.
This exceptionally strong and specific binding has made the biotin-avidin system a widely used tool in molecular
The biotin-avidin system is employed in numerous techniques, including enzyme-linked immunosorbent assays (ELISAs), Western blotting, immunohistochemistry,