agarosegelelektroforesen

Agarose gel electrophoresis is a laboratory technique used to separate and analyze DNA, RNA, or proteins based on their size and charge. The process involves the use of agarose, a type of polysaccharide derived from seaweed, which is melted and poured into a mold to form a gel. The gel is then placed in an electrophoresis chamber, and an electric field is applied. The molecules to be analyzed are mixed with a loading dye and loaded into wells at one end of the gel. When the electric field is activated, the molecules migrate through the gel towards the opposite end. Smaller molecules move faster and travel further, while larger molecules move slower and travel shorter distances. The separated molecules are then visualized using staining techniques or fluorescent dyes. This technique is widely used in molecular biology and genetics for tasks such as DNA sequencing, gene mapping, and protein analysis.