Verdunningsreeks

A verdunningsreeks, also known as a dilution series, is a sequence of solutions prepared by progressively diluting a stock solution with a solvent. This technique is commonly used in various scientific and analytical procedures to create a range of concentrations for calibration, standardization, or testing purposes. The process involves taking a known volume of the stock solution and adding a known volume of the solvent to achieve the desired dilution factor. This is repeated to create a series of solutions with increasing dilution.

The dilution factor is calculated by dividing the final volume of the solution by the initial volume of the stock solution. For example, if 1 mL of a stock solution is diluted to 10 mL with solvent, the dilution factor is 10. The concentration of the diluted solution can be determined using the formula: C1V1 = C2V2, where C1 and C2 are the concentrations of the stock and diluted solutions, respectively, and V1 and V2 are the volumes of the stock and diluted solutions, respectively.

Verdunningsreeks are widely used in various fields, including chemistry, biology, and environmental science. They are particularly useful in spectrophotometry, where a series of diluted solutions is used to create a standard curve for quantitative analysis. In microbiology, dilution series are used to enumerate microorganisms in a sample. In environmental science, they are used to analyze the concentration of pollutants in water or soil samples. The preparation of a verdunningsreeks requires careful measurement and mixing to ensure accurate and reproducible results.