Standardkõverate

Standardkõverate, often translated as "standard curves" or "calibration curves," refers to a graphical representation used in analytical chemistry and other scientific fields to determine the concentration of an unknown analyte. This curve is created by plotting a series of known concentrations of a substance against their corresponding measured signal, such as absorbance, fluorescence, or peak area from a chromatographic separation. The measured signal is typically plotted on the y-axis, and the known concentration is plotted on the x-axis.

The fundamental principle behind a standard curve is that there is a reproducible relationship between the concentration of an analyte and the instrument's response. Once a sufficient number of data points are generated from standards of known concentrations, a mathematical model, often a linear regression line, is fitted to these points. This fitted line represents the calibration.

To determine the concentration of an unknown sample, the same analytical procedure used for the standards is applied to the unknown. The resulting signal from the unknown sample is then located on the y-axis of the standard curve. By tracing horizontally from this signal to the calibration line and then vertically down to the x-axis, the corresponding concentration of the analyte in the unknown sample can be read. Standard curves are essential for quantitative analysis, ensuring accuracy and reliability in experimental results across various scientific disciplines.