Rekellularisierung

Rekellularisierung refers to the process of reintroducing cells into a de-cellularized scaffold. This technique is a crucial step in tissue engineering, aiming to create functional biological substitutes for damaged or diseased tissues and organs. The process begins with de-cellularization, where the cellular components are removed from a donor tissue or organ, leaving behind a three-dimensional extracellular matrix that retains the original structural and biological cues. Following de-cellularization, the empty scaffold is then repopulated with specific cell types, either autologous (from the patient), allogeneic (from a donor of the same species), or even engineered cells.

The goal of re-cellularization is to restore the cellular architecture and function of the original tissue. The choice of cell type, cell density, and delivery method are critical factors influencing the success of this process. Different seeding techniques, such as static seeding, dynamic perfusion, or bio-printing, are employed to ensure even cell distribution and promote cell adhesion and proliferation within the scaffold. After cell seeding, the construct is typically cultured in a bioreactor under controlled conditions to allow for cell growth, differentiation, and the formation of new tissue. Rekellularisierung holds significant promise for regenerative medicine, offering potential solutions for organ transplantation shortages and the treatment of various degenerative diseases.