RPMI1640stä
RPMI 1640, short for Roswell Park Memorial Institute medium 1640, is a buffered, isotonic cell culture medium widely used for in vitro growth of mammalian cells. Developed at Roswell Park Memorial Institute in the 1960s, it became a standard base medium for immunology and cell biology research, particularly for lymphoid cell lines, hybridomas, and various suspension cultures.
The formulation is designed to support the growth of a broad range of mammalian cells and contains
Variants of RPMI 1640 include formulations with or without L-glutamine, and with additional buffering or energy
Applications include routine culture of lymphocytes and hematopoietic cells, growth of various immortalized cell lines, and