Nonautoclaved

Nonautoclaved refers to materials, equipment, or specimens that have not been sterilized by autoclaving. Autoclaving uses saturated steam under pressure to inactivate microorganisms, typically at 121°C for a defined period. The term signals that a given item has not undergone that specific sterilization step, although it does not by itself confirm sterility or contamination.

In laboratory and medical settings, autoclaving is a common method for sterilizing glassware, culture media, liquids, and certain waste streams. Some items are not autoclaved intentionally because their contents are heat sensitive, moisture sensitive, or intended for use in nonsterile workflows. Examples include heat-labile enzymes, some chemical reagents, and certain prepackaged items that rely on other barriers for sterility.

Alternatives to autoclaving for achieving sterility or decontamination include filtration of liquids through 0.22-micron or smaller membranes, chemical sterilants such as ethylene oxide or hydrogen peroxide vapor, dry heat sterilization, and irradiation. In food processing or consumer products, nonautoclaved labeling can indicate products that are raw, minimally processed, or otherwise not subjected to steam sterilization.

Handling and safety considerations: Nonautoclaved items that may harbor infectious agents or contaminants require appropriate biosafety measures. Waste from nonautoclaved sources often must be disinfected or disposed of according to regulatory guidelines before handling or disposal.

See also

Autoclave, Sterilization, Filtration, Ethylene oxide.