NascentStrandSequencing
Nascent strand sequencing is a molecular biology technique used to study newly synthesized RNA molecules directly within a cell. It allows researchers to capture and analyze RNA that is actively being transcribed from DNA, providing insights into gene expression dynamics and regulatory mechanisms. The process typically involves a short labeling period where cells are exposed to modified nucleotides that are incorporated into newly forming RNA strands. These modified nucleotides act as a tag.
Following the labeling, the RNA is extracted from the cells. The key step in nascent strand sequencing
The resulting sequence data provides information about the location and abundance of nascent RNA transcripts. This