NADPdependent
NADP-dependent refers to enzymes that catalyze reactions using NADP+ as a cofactor, typically yielding NADPH in the process. These enzymes have specificity for the phosphorylated form of the dinucleotide; some enzymes can utilize either NADP+ or NAD+ and are described as NAD(P)-dependent.
In metabolism, NADPH acts as a reducing equivalent, supporting anabolic biosynthesis and defense against oxidative stress.
Prominent examples include glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase in the pentose phosphate pathway; NADP+-dependent isocitrate
Molecular recognition: Many NADP+-dependent enzymes have a Rossmann fold with a binding pocket that recognizes the
Physiological relevance: NADP+-dependent NADPH production is essential for redox homeostasis, fatty acid and cholesterol synthesis, and