Mobiilfaasi

Mobiilfaasi refers to the mobile phase in chromatography. In this separation technique, the mobile phase is the liquid or gas that carries the sample mixture through the stationary phase. The mobile phase's properties, such as its polarity, viscosity, and flow rate, are critical factors in determining the efficiency and selectivity of the separation. Different types of chromatography employ different mobile phases. For instance, in liquid chromatography, common mobile phases include mixtures of water, organic solvents like methanol or acetonitrile, and sometimes buffers. In gas chromatography, the mobile phase is an inert gas, such as helium, nitrogen, or hydrogen. The interaction between the mobile phase and the components of the sample, as well as their interaction with the stationary phase, dictates how quickly each component moves through the system. Components that are more soluble or have weaker interactions with the stationary phase will elute faster, while those with stronger interactions or lower solubility in the mobile phase will be retained longer. Optimization of the mobile phase composition is a key aspect of method development in chromatography to achieve the desired separation of analytes.