MikrotomSchneidung

MikrotomSchneidung refers to the process of preparing tissue samples for microscopic examination. This technique involves sectioning extremely thin slices of biological material, typically ranging from 0.5 to 10 micrometers in thickness. These thin sections are essential for light microscopy, as they allow light to pass through the tissue, enabling visualization of cellular structures.

The process is carried out using a precision instrument called a microtome. The tissue sample is first embedded in a supporting medium, such as paraffin wax or frozen within a cryostat. The embedded block is then mounted onto the microtome, and a very sharp blade, often made of steel or glass, is used to cut the specimen into uniform, thin sections. These sections are then transferred to microscope slides, stained with specific dyes to highlight different cellular components, and finally covered with a coverslip for observation under a microscope.

MikrotomSchneidung is a fundamental technique in various scientific fields, including histology, pathology, and research. It is crucial for diagnosing diseases by allowing pathologists to examine tissue abnormalities, and it plays a vital role in scientific research for understanding cellular biology and the effects of various treatments or conditions on tissues. The quality of the microtome sections directly impacts the clarity and diagnostic accuracy of the subsequent microscopic analysis.