LectinAssays
LectinAssays are laboratory techniques that utilize lectins—carbohydrate-binding proteins—to detect and analyze glycosylation patterns on glycoproteins, cell surfaces, or other glycoconjugates. Lectins exhibit selective affinities for certain monosaccharides and linkages, such as galactose, mannose, fucose, sialic acid, and N-acetylglucosamine, though binding can be influenced by linkage and context.
Common formats include ELISA-based lectin assays, where a capture molecule binds the target and a labeled lectin
Key considerations include lectin selection based on the target glycan motif, ligand density, calcium or other
Applications span glycomics, development of glycoprotein therapeutics, cancer and autoimmune disease research, and quality control in