HPLCn
HPLCn, or High-Performance Liquid Chromatography with n-dimensional separation, is an advanced analytical technique used in chemistry and biochemistry to separate, identify, and quantify each component in a mixture. It is an extension of traditional High-Performance Liquid Chromatography (HPLC), which typically uses a single column for separation. In HPLCn, multiple columns with different separation mechanisms are employed in series or in parallel, significantly enhancing the separation power and resolution.
The 'n' in HPLCn denotes the number of dimensions or columns used in the separation process. For
HPLCn is particularly useful in proteomics, metabolomics, and other fields where the analysis of complex biological
The main challenge in HPLCn is the compatibility of the different separation mechanisms used in each dimension.