FRETmittaukset
FRETmittaukset, or Förster Resonance Energy Transfer measurements, are a biophysical technique used to study molecular interactions and distances. It relies on the phenomenon of non-radiative energy transfer between two light-sensitive molecules called fluorophores. One fluorophore, the donor, absorbs light at a specific wavelength and, if it is sufficiently close to a second fluorophore, the acceptor, it can transfer this energy to the acceptor without emitting a photon. The acceptor then emits light at its characteristic wavelength.
The efficiency of FRET is highly dependent on the distance between the donor and acceptor fluorophores, typically
Applications of FRET include studying protein-protein interactions, conformational changes in biomolecules, and the dynamics of molecular