F3hydroksylaza

F3hydroksylaza, also known as flavanone 3-hydroxylase, is an enzyme that plays a crucial role in the biosynthesis of flavonoids in plants. This enzyme belongs to the 2-oxoglutarate-dependent dioxygenase superfamily. Its primary function is to catalyze the hydroxylation of flavanone at the 3-position. This reaction is a key step in the formation of flavonols, a subclass of flavonoids known for their diverse biological activities, including antioxidant and UV-protective properties.

The gene encoding F3hydroksylaza is often referred to as the TT3 gene in *Arabidopsis thaliana*. Mutations in this gene can lead to altered flavonoid profiles, resulting in visible phenotypes such as changes in flower color or leaf pigmentation. The enzyme requires 2-oxoglutarate, iron, and molecular oxygen as cofactors for its activity. It is typically found in the cytosol of plant cells.

F3hydroksylaza is an essential component of the flavonoid biosynthesis pathway, linking the production of flavanones to that of flavonols. This pathway is vital for plant development, defense against pathogens, and adaptation to environmental stress. Understanding the activity and regulation of F3hydroksylaza is therefore important for research in plant biochemistry, genetics, and agricultural applications.