Enkelbølgelengdefotometre

Enkelbølgelengdefotometre, sometimes referred to as single-wavelength photometers, are instruments designed to measure the intensity of light at a specific, narrow band of wavelengths. This specificity allows for the isolation and measurement of a particular component or characteristic within a sample that absorbs or emits light at that chosen wavelength. Unlike broadband photometers that measure across a wide spectrum, single-wavelength instruments focus on a singular point in the electromagnetic spectrum.

The operation typically involves a light source, a wavelength selection mechanism (such as a filter or monochromator), a sample holder, and a detector. The light source emits radiation, which then passes through the wavelength selector to isolate the desired wavelength. This monochromatic light interacts with the sample, and the detector measures the transmitted or emitted light. The difference between the incident and transmitted light intensity, or the intensity of emitted light, provides quantitative information about the substance being analyzed.

Single-wavelength photometers are employed in various scientific and industrial applications. They are commonly used in chemistry for quantitative analysis, such as determining the concentration of specific ions or molecules in solutions. In biology, they can be used for measuring the concentration of proteins or nucleic acids. Environmental monitoring also utilizes these devices for assessing pollutant levels in water or air. Their simplicity and accuracy at a specific wavelength make them valuable tools for routine measurements and specialized analytical tasks.