DurchflusszytometrieAnalyse

Durchflusszytometrie, often abbreviated as flow cytometry, is a powerful laboratory technique used to detect and measure physical and chemical characteristics of a cell or particle. It operates by suspending cells in a fluid stream and passing them one by one through a laser beam. As each cell passes through the laser, it scatters light, and fluorescent markers that have been attached to specific cellular components emit light at different wavelengths. Detectors measure the intensity and wavelength of this scattered and emitted light.

This data is then analyzed to identify and quantify different cell populations within a sample. Key applications of flow cytometry include cell counting, cell sorting, and immunophenotyping, where specific cell surface markers are identified. It is widely used in research, clinical diagnostics, and drug discovery. For example, it can be used to diagnose leukemia and lymphoma by identifying abnormal white blood cells, or to monitor immune system health. The technology allows for the analysis of thousands of cells per second, providing a rapid and detailed snapshot of a cell population.